FastQCFastQC Report
Sun 19 Mar 2023
SRR4896097_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896097_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593984
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT18460.3107827820277987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTGA594750.0144.717792
GAATCTG595050.0144.644821
ATCTGAG212850.0144.470153
ATCTGAA162100.0144.286933
ATCTGAT211050.0144.226073
TCTGAGT80300.0143.829914
CTGATGT25600.0143.780585
TCTGATG76650.0143.305714
CTGATAG21900.0143.258455
TCTGAAG56500.0143.245564
TCTGAGG73850.0143.244784
CTGAATG21400.0143.21985
TCTGAAT60150.0142.744434
TCTGAGA58700.0142.690874
CTGAGTA25300.0142.62165
TCTGATA65700.0142.26594
CTGATTG23650.0142.155365
CTGAAGT19600.0141.585625
CTGAGAG16600.0141.420845
CTGAGGT25000.0141.144975
Produced by FastQC (version 0.11.5)