FastQCFastQC Report
Sun 19 Mar 2023
SRR4896096_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896096_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565153
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCAT1566650.0144.763061
TGGCATA1567250.0144.707642
GGCATAT568300.0144.455613
GGCATAG501300.0144.453773
GCATATG194400.0144.230964
GGCATAA470700.0144.10143
GCATAAG162600.0143.96614
GCATAGT181700.0143.665714
GCATAGG167300.0143.602624
GCATAGA150950.0143.50994
GCATAAT168600.0143.139944
GCATATT199450.0142.649634
GCATATA175200.0142.544664
CATATGG64350.0141.974495
CATAAGT55850.0141.13985
CATAGGT55350.0141.10585
CATATAG60200.0141.05065
CATAAGG55100.0140.95715
CATAGAG44750.0140.368565
CATAATG57500.0140.365745
Produced by FastQC (version 0.11.5)