FastQCFastQC Report
Sun 19 Mar 2023
SRR4896094_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896094_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005399
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG2008250.0144.702091
TCAATGA2009400.0144.619252
CAATGAG654200.0144.29113
CAATGAT722900.0144.179663
CAATGAA605400.0143.104143
AATGATG265850.0140.332614
AATGAGT244700.0139.936844
AATGAGG238800.0139.207124
AATGAAG213500.0138.531174
AATGAGA188050.0138.20714
CAATGAC39050.0136.747313
AATGATA224800.0136.047764
AATGAAT233100.0134.747044
AATGATT265100.0134.252084
ATGAGTG80250.0134.076865
ATGATAG79050.0132.995795
ATGATGG89850.0132.331395
ATGAATG85050.0131.280645
ATGATGT100950.0131.27435
ATGAGAG58950.0131.26835
Produced by FastQC (version 0.11.5)