FastQCFastQC Report
Sun 19 Mar 2023
SRR4896093_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896093_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352281
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCATACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT33010.9370360592822208No Hit
CGCATACATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT7770.22056256227273113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGC253.7200152E-8144.910438
CGCATAC352600.0144.766591
GCATACA352850.0144.664022
CATACAG118700.0143.872743
CATACAT124150.0143.859943
ATACATG49050.0143.137824
ATACAGT41900.0142.662434
TACATGA12700.0142.628375
ATACAGG40250.0142.570274
ATACAGA36450.0141.729974
CATACAA103750.0141.628133
TACAGTA12000.0140.683885
ACAGATG3400.0140.648366
TACAGTG13400.0140.584755
TACAGGT14450.0140.397645
CAGTAGG1600.0140.381997
ACAGTAG4200.0139.735066
TACAGAT12700.0139.205295
TACAGAG12200.0138.971485
TACATGT21200.0138.075045
Produced by FastQC (version 0.11.5)