FastQCFastQC Report
Sun 19 Mar 2023
SRR4896092_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896092_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220322
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTCA2223450.0144.715582
AGTGGTC2226300.0144.530321
TGGTCAG835900.0144.496123
TGGTCAT680050.0144.270923
TGGTCAA667500.0144.215293
GGTCATG281400.0144.09694
GGTCAGT274000.0143.81024
GGTCATA201850.0143.520864
GGTCAAT208650.0143.32344
GGTCAGA241650.0143.301674
GGTCAAG265100.0143.19894
GGTCAGG321100.0142.889914
GTCATGA75550.0142.619035
GGTCATT195750.0142.107624
GTCATAG81550.0142.077565
GTCAGTA78450.0142.057575
GTCAGGT99450.0142.006445
GTCAGAT76500.0141.795155
GTCAATG88900.0141.497565
GTCAAGT78800.0141.058785
Produced by FastQC (version 0.11.5)