FastQCFastQC Report
Sun 19 Mar 2023
SRR4896092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2220322
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA184820.8324017867678652No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG169620.7639432478712547No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG161360.7267414366024387No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC108250.48754189707618983No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGTTTCAAAGTGGAGAACC105060.47317461161038804No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT32740.14745608970230445No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23770.10705654405081787No Hit
TGTCTTGATGACTGCTGATTTTCTGGAATGGAAAATTAAGTTGTTTAGTG22600.10178703809627612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT376550.0111.691131
GGTATCA381250.0110.367938
GTATCAA381450.0110.115039
GTGGTAT381250.0109.74046
TGGTATC382150.0109.633727
AGCAGTG385250.0109.074732
CAGTGGT391600.0107.065314
AGTGGTA394950.0105.9568565
GCAGTGG396800.0105.5891343
AAATATA53350.049.32846145
CGGTCTG5300.035.565145
CGCCGAA1656.023947E-835.15049145
CGGTATC1601.8789106E-631.7178237
CGCTGTT6450.031.471949145
AGTGGTC11550.027.623841
CAGCGCA37200.025.84245345-49
GAAACCG37300.025.61871585-89
AGGTATC4250.025.5874887
AAACCGC37450.025.51610485-89
AGCGCAG38000.025.26024445-49
Produced by FastQC (version 0.11.5)