FastQCFastQC Report
Sun 19 Mar 2023
SRR4896091_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896091_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94510
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACTGGAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT1560.16506189821182946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCA151.2288128E-4144.93179
TGCGTCA100.007090752144.93179
GATACTG100.007101986144.855037
ATTAGAG100.007101986144.855038
GAATGCT100.007101986144.855037
GAGTCGA100.007101986144.855037
TGGACGT100.007101986144.855035
TGGACGA151.2314081E-4144.855035
TGGACAG306.493792E-10144.855035
ATGTGTC151.2314081E-4144.855038
GCGATCG100.007101986144.85503145
GATGAAG306.493792E-10144.855037
CTGGATC351.0913936E-11144.855034
CTGGAGT11250.0144.855034
AGTCGGA100.007101986144.855038
CGACTGG94550.0144.778431
ACTGGAT32550.0144.632523
GACTGGA94650.0144.625462
ACTGGAG31600.0144.396623
CTGGATT11650.0143.611634
Produced by FastQC (version 0.11.5)