FastQCFastQC Report
Sun 19 Mar 2023
SRR4896090_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896090_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474757
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAACTTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11390.23991220771889618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT475800.0144.591841
GAACTTA476250.0144.455222
AACTTAG158850.0144.364293
ACTTAGG54850.0143.986954
ACTTAGA45550.0143.320954
ACTTATG60350.0142.990684
ACTTAAG51050.0142.640754
ACTTAGT58350.0142.428154
AACTTAT173750.0141.867453
CTTAGGT17000.0139.79715
CTTAGAG14900.0139.076265
CTTAGGG18450.0139.020925
CTTATAG19500.0138.966545
CTTATGG20600.0138.932285
AACTTAA143500.0138.751623
CTTAGTG18500.0138.645195
ACTTATA55550.0137.868234
CTTAAGG19150.0137.722815
CTTAGGA16850.0137.171555
CTTAGTA18450.0137.057345
Produced by FastQC (version 0.11.5)