FastQCFastQC Report
Sun 19 Mar 2023
SRR4896090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474757
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG117522.475371611161078No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG103372.177324399640237No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC47871.0083053014489518No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31080.6546506949871197No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA16860.3551290449640553No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTTACCTCGTTGCACTGC8760.18451544684965152No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC6790.14302053471565454No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC6430.13543770813279216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5850.12322093197151385No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT185950.0100.209451
GTATCAA192200.097.355539
AGCAGTG191550.097.2522052
GTGGTAT191000.097.180336
GGTATCA193100.096.836888
TGGTATC192950.096.19827
CAGTGGT193100.096.0584954
GCAGTGG196250.094.664463
AGTGGTA198400.093.59225
AAATATA21700.069.49849145
TTGCGTA350.003564252362.147686145
CGAACTT1154.0017767E-1156.743541
CGCAAGG3150.046.035324145
GGTCTGA2950.039.32509145
GGGGTAT1604.5778506E-836.252826
AGGGGTA1604.5778506E-836.252825
AGGTATC2051.1350494E-831.8317437
CGGTATC1255.14747E-429.0022547
GGCCGAC1000.006068982629.002253145
GCGGTAT1306.4749515E-427.8867846
Produced by FastQC (version 0.11.5)