FastQCFastQC Report
Sun 19 Mar 2023
SRR4896089_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896089_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499135
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT33540.6719624951165517No Hit
CCTCCTGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT7680.15386618850611558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGT100.007100422144.927449
CTCCTGA500050.0144.608642
TCCTGAT176350.0144.308793
TCCTGAG169800.0144.115943
CCTGATG60750.0143.929924
TCCTGAA143750.0143.825593
CCTGAGT67800.0143.494894
CTGATAG18350.0143.304785
CCTCCTG504900.0143.219541
CCTGATA53850.0143.000534
CTGAGTA17100.0142.76575
CTGATTG19900.0142.699725
CCTGATT60700.0142.616354
CCTGAAT52400.0142.118934
CTGATGG19750.0141.949545
CCTGAGA46150.0141.901464
CCTGAAG49150.0141.788714
CTGAGTT25350.0141.740455
CTGAATG19050.0141.461445
CCTGAGG56350.0141.155734
Produced by FastQC (version 0.11.5)