FastQCFastQC Report
Sun 19 Mar 2023
SRR4896089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499135
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG236574.739599507147365No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG55401.1099201618800525No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39700.7953760004808318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA30850.6180692598194877No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC25130.5034710048383704No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6670.13363118194476445No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG5190.1039798852013984No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG100.0070900256144.99849145
AAGCAGT389050.0105.979531
GTATCAA397250.0103.97979
GGTATCA397850.0103.762968
AGCAGTG398150.0103.34912
GTGGTAT397100.0103.319816
TGGTATC398500.0103.011427
AGTGGTA399150.0102.8176355
CAGTGGT399400.0102.807754
GCAGTGG404950.0101.380823
AGGTATC2650.049.2546427
GGGTATC2550.048.3425187
GGGGTAT2600.047.4128576
AAATATA20100.046.89006145
AGGGGTA2700.045.66145
GAGGTAT3350.038.9626276
GTTTACG951.0282137E-438.157497145
CAGGGGT3500.035.2245064
CCGCAAG1801.2903183E-732.22189145
AGAGGTA4100.031.838515
Produced by FastQC (version 0.11.5)