FastQCFastQC Report
Sun 19 Mar 2023
SRR4896088_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896088_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634244
Sequences flagged as poor quality0
Sequence length151
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAGA634950.0144.72392
CCGTGAG635050.0144.701131
GTGAGAG203950.0143.438643
GTGAGAT224800.0143.348143
GTGAGAA200450.0143.087943
TGAGATG78350.0143.045734
TGAGAGT71450.0142.157384
TGAGAGA55850.0141.133264
TGAGAAT70850.0140.804894
TGAGATA71850.0140.55934
TGAGAAG71900.0139.655474
TGAGAGG78200.0139.429084
TGAGATT76200.0139.380684
TGAGAAA63950.0132.886574
GAGATTA27050.0132.84285
GAGATGG27300.0131.626285
GAGATTG25550.0131.0015
GAGATAG25300.0130.577365
GTGAGAC13300.0130.187673
GAGAGTA25350.0129.462455
Produced by FastQC (version 0.11.5)