FastQCFastQC Report
Sun 19 Mar 2023
SRR4896088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634244
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG95221.5013149513436468No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52880.8337485258039493No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG50380.7943315190999047No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC28660.45187656485516614No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA18220.2872711448590763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA15830.24958848645000978No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13120.20686045118282556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTCTATGTTCATTCTATA9170.14458158059043522No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTTAGTTTGTGGCTTCAT7830.12345406499706738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT254950.0118.926621
GGTATCA259200.0117.3590858
GTATCAA259950.0116.964919
GTGGTAT259000.0116.889756
AGCAGTG259400.0116.848962
TGGTATC260350.0116.4229057
CAGTGGT261550.0115.777514
AGTGGTA262150.0115.512535
GCAGTGG265050.0114.166573
GGGTATC2650.046.5181857
AGGGGTA2600.044.634345
AAATATA19050.040.339252145
AGGTATC2650.038.3090937
AGCGCAT951.0287584E-438.156048145
TAGGTTC800.002036537736.24825145
AGACTCG850.002741882634.115997145
GGGGTAT3500.033.1490946
CGTGAGA1102.4290834E-432.9685482
GTATCCA3300.032.9529539
CGCACTA900.003628097232.220665145
Produced by FastQC (version 0.11.5)