FastQCFastQC Report
Sun 19 Mar 2023
SRR4896087_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896087_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253531
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG151.2312642E-4144.930977
ATAGCGG100.007097861144.930958
AGCTAGA100.007097861144.930959
TAAGCTA202.132556E-6144.930957
AGTCGAC100.007097861144.930958
CGAAGTA253650.0144.845262
CCGAAGT253850.0144.731141
GAAGTAG83900.0144.153633
GAAGTAT87350.0143.935443
GAAGTAA78900.0143.645143
AAGTAGG31300.0142.615774
AAGTAGT29550.0142.233434
AAGTAAG26900.0140.890144
AAGTATG31350.0140.770264
GTAGTAG2800.0139.754876
AAGTAGA23900.0139.473334
GAAGTAC5300.0139.461883
AAGTAAT29050.0138.694694
AGTAGTA10100.0137.038685
AGTAATG9950.0136.919715
Produced by FastQC (version 0.11.5)