FastQCFastQC Report
Sun 19 Mar 2023
SRR4896087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253531
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG145345.732632301375374No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30771.2136582903076942No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA19000.749415258883529No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG13040.5143355250442747No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCCCGAGCCGCCTGGATA8760.34551987725366917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA5680.22403571949781287No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACCGCCCGTCCCCGCCCCTT5430.21417499240724017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAGTGTTCAAAGCAGGCCC5330.21023070157101106No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACGAGGATCCATTGGAGGG4200.1656602151216222No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3950.15579948803104945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGCCGCCTGGATACCGCAG3930.15501062986380362No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG3440.13568360476628105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGAAACCCTGTCTCTA2970.11714543783600427No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2780.10965128524716898No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTCCAATAGCGTATATTA2640.10412927807644824No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGACGAAAATGGAAAGATTA2630.10373484899282534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT240750.0106.289311
AGCAGTG248450.0102.8873142
GTATCAA248600.0102.854399
GGTATCA249000.0102.630938
GTGGTAT248600.0102.241996
TGGTATC249100.0102.094977
CAGTGGT249150.0102.0453954
AGTGGTA250200.0101.5881655
GCAGTGG254000.0100.410843
ATAGGGG451.2305518E-680.55194145
ACGAATA300.001934414772.49674145
AAGCGTA350.003564067962.140068145
ACGGGCA400.006046771554.37256145
CGAGCCC400.006046771554.37256145
CGGTCGC400.006046771554.37256145
CCGGCCG553.215125E-452.724903145
AGGGGTA1650.052.7249035
CACCAGG1600.049.84151145
ATTGCGC3050.047.53885145
GCTAGTT1109.923315E-846.134293145
Produced by FastQC (version 0.11.5)