FastQCFastQC Report
Sun 19 Mar 2023
SRR4896086_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896086_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146863
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTTCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT57303.9015953643872177No Hit
CCAGTTCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT9230.6284768798131591No Hit
CCAGTTCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT7050.4800392202256525No Hit
CCAGTTCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT6440.43850391180896486No Hit
CCAGTTCAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT1800.12256320516399637No Hit
CCAGTTCATGTACTCTGCGTTGATACCACTGCTCCCCACGTACTCTGCGT1700.11575413821044102No Hit
CCAGTTCATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCGT1620.11030688464759675No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGG253.716741E-8144.86117
CAAGTCG151.2325833E-4144.861087
CATGCTA100.007105078144.861087
CATGCGA151.2325833E-4144.861087
TTGCTAG100.007105078144.861089
CAGTTAG450.0144.861087
ATTCGTC100.007105078144.861088
ATGCTGT100.007105078144.861088
CATTCGT202.1346532E-6144.861087
TCAGGAA600.0144.861086
AGTATGA151.2325833E-4144.861088
GTCAGGT100.007105078144.861089
TCAGATG306.511982E-10144.861086
GTCGTAT202.1346532E-6144.861089
CATACGG202.1346532E-6144.861087
CATGTGA450.0144.861087
CATAAGT550.0144.861087
CATAAGG750.0144.861087
CAGTAGT750.0144.861087
AGTCGTC100.007105078144.861088
Produced by FastQC (version 0.11.5)