FastQCFastQC Report
Sun 19 Mar 2023
SRR4896086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146863
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG2365716.108209692025902No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA32682.22520308042189No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19111.3012126948244283No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA7200.49025282065598547No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA5700.3881168163526552No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5630.3833504694851665No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG5240.3567951083663006No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG4570.3111743597774797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT4040.27508630492363634No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTCCAATAGCGTATATTA3800.25874454423510346No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC3180.21652832912306025No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2730.18588752783206117No Hit
AAGCAGTGGTATCAACGCAGAGTACATGAACTGGAGATCGGAAGAGCACA2340.1593321667131953No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAGTGGTATCAACGCAG2270.15456581984570653No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCTTAAAGTTTAGTTTAAA2160.14707584619679565No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACGAGGATCCATTGGAGGG2060.1402667792432403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGAATTGACTTGGACCAT1990.13550043237575154No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT1940.13209589889897388No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1930.13141499220361835No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG1920.13073408550826282No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC1790.12188229846864085No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1730.11779685829650763No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTCAGGTTTAAGGCTTTG1720.1171159516011521No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATTTTCCTTTTTTCTTCA1710.11643504490579655No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGAAACCCTGTCTCTA1660.11303051142901888No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC1640.11166869803830781No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAAAAAGACAAGCTA1620.11030688464759675No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG1580.1075832578661746No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT1570.10690235117081907No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG1560.10622144447546354No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAATTCTGCCCCCTTTTGT1530.10417872438939692No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG1520.10349781769404139No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATTGGACCAATCTATCACC1520.10349781769404139No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACTAAACGCAGACGAAAATG1490.10145509760797478No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGC100.007094156144.93565145
AAGCAGT201700.079.393131
AGCAGTG209750.076.285562
AGTGGTA212850.075.106425
GTATCAA214550.075.085529
GGTATCA215100.074.9609158
CAGTGGT213950.074.754144
GTGGTAT215300.074.319076
GCAGTGG215650.074.131243
TGGTATC216050.074.094617
AGGGGTA1250.063.7716875
CCGGCTC808.050847E-963.409344145
CATGCGA350.003566715462.115276145
TAACAGG350.003566715462.115276145
CAGGCGA350.003566715462.115276145
AGCTAGT350.003566715462.115276145
CCGCAAG753.7504833E-757.974262145
GAATCCA502.0089792E-457.97426145
GGGGTAT1450.054.975596
GGGTATC1500.053.1430747
Produced by FastQC (version 0.11.5)