FastQCFastQC Report
Sun 19 Mar 2023
SRR4896085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349111
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6556818.781419090203403No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA82322.3579892928037216No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG30630.877371380449198No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25160.7206876895886982No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC20740.594080392769062No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA15150.43395939973246334No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA6830.19563978218961878No Hit
AAGCAGTGGTATCAACGCAGAGTACATTGGTATGAGATCGGAAGAGCACA5650.16183964412464805No Hit
AGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG5380.15410571422842592No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5070.1452260169401709No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4600.13176325008378423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG4340.12431576203557033No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4320.1237428783395539No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG4300.12316999464353745No Hit
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT4250.12173778540349632No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAG4150.11887336692341405No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT4150.11887336692341405No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4050.11600894844333176No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAACGAGGATCCATTGGAGGG3940.11285808811524128No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC3600.10311906528296158No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT3570.10225973973893689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT510200.067.146141
AGCAGTG524050.065.357292
GTATCAA529900.064.859079
GGTATCA530800.064.762768
AGTGGTA527350.064.755825
CAGTGGT529450.064.5263754
GTGGTAT529900.064.4486546
TGGTATC531000.064.383417
GCAGTGG533800.064.0276953
AGGGGTA1900.053.4233745
AAATATA10150.050.709225145
GACCGAG450.0096377248.32851145
GGGGTAT2150.047.2045946
TCCGCAT657.3336007E-444.610935145
CGTGCGC1002.7308797E-643.49566145
GGGTATC2500.040.595957
GACTATG907.476818E-540.273758145
CATGCGC1105.2580526E-639.54151145
CTCGGCG1651.3624231E-939.541508145
CACGGGC1157.134251E-637.822315145
Produced by FastQC (version 0.11.5)