FastQCFastQC Report
Sun 19 Mar 2023
SRR4896084_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896084_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194032
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT2150.0144.898996
CGAGTTA1500.0144.898976
AGAACGT100.007101272144.898978
AGTGCGT100.007101272144.898978
CGAATGC151.231905E-4144.898976
CGAGATT950.0144.898976
CGAGATG2950.0144.898976
CGATGAG2450.0144.898976
TCGAGCG306.511982E-10144.898975
TCGAGAT5600.0144.898975
CGATCGT202.1336236E-6144.898976
CGATCAA151.231905E-4144.898976
TCGACTA202.1336236E-6144.898975
CGATAAT2000.0144.898976
ACGCGTC100.007101272144.898978
ACTTCGA194200.0144.749762
CACTTCG194200.0144.749761
CTTCGAG63450.0144.328053
CTTCGAT72250.0144.096763
TTCGATA22350.0143.92654
Produced by FastQC (version 0.11.5)