FastQCFastQC Report
Sun 19 Mar 2023
SRR4896084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194032
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG77083.972540611857838No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27421.4131689618207306No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10370.5344479261152799No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA7060.36385750803991096No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG4820.24841263296775787No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCCTGGCCAACATGGTGAA2810.14482147274676344No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGAAACCCCGTCTCTA2680.13812154696132595No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATCCCAGCACTTTGGGAGG2560.13193700008246062No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2450.1262678321101674No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAGATTTATAGGTAGAG2230.11492949616558094No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA2150.11080646491300405No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCCAAGTGAACAAAGCACC2110.1087449492867156No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGAACCTATGGGTCGTG1980.10204502350127814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT154300.0112.551651
AGCAGTG158600.0109.426162
CAGTGGT159050.0108.8885654
AGTGGTA159100.0108.7807165
CGGGTAA203.864151E-4108.7299145
GTGGTAT159050.0108.695716
GGTATCA159550.0108.673118
TGGTATC159500.0108.34367
GTATCAA160150.0108.039669
GCAGTGG160900.0107.636583
CCCGTTG300.001934801372.4866145
GGGGTAT902.8012437E-1064.432536
AGGGGTA1051.1004886E-955.242125
GGGTATC1051.1023076E-955.2278827
GTATCAC1203.601599E-948.32449
ATAGCTC657.3285843E-444.60714145
CAGATTG1903.6379788E-1241.965927145
TAGACTG750.001481783138.65952145
TAATTAC750.001481783138.65952145
AGCAGGG1801.2795681E-732.232882
Produced by FastQC (version 0.11.5)