FastQCFastQC Report
Sun 19 Mar 2023
SRR4896083_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896083_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174870
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTAGC100.0071020466144.889628
CAATGGA175400.0144.435291
AATGGAA176900.0143.210572
TGGAATG23700.0143.055574
ATGGAAT65750.0142.796163
ATGGAAG55700.0142.54853
TGGAAGT20150.0142.372924
TGGAATA19600.0141.563084
ATGGAAA52850.0140.91443
TGGAAAT20900.0139.690234
TGGAAAG17000.0138.497444
TGGAAGG21300.0138.087284
GGAATAG5300.0136.688325
TGGAAGA14750.0136.540054
TGGAATT22900.0135.399034
GGAATGA6100.0134.201055
GGAATTG7050.0133.586175
GGAAGTA6300.0133.390465
ATGGAAC4200.0132.815493
GGAAGTG7100.0132.645435
Produced by FastQC (version 0.11.5)