FastQCFastQC Report
Sun 19 Mar 2023
SRR4896083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174870
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG133837.653113741636644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18561.0613598673300166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA17220.9847315148395951No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7820.44718934065305654No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC7640.4368959798707612No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3460.19786126837078974No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGGGAATAAGCATAACC3310.18928346771887689No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGAAACCCCGTCTCTA2800.16011894550237318No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA2630.15039743809687195No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGTGATTAAAATAATTTGA2490.14239149082175329No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGATTAAAATGAAGGTGACT1850.10579287470692514No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGAAACCCCATCTCTA1770.10121804769257163No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAGTTTAGTTTAAAAGTTG1760.10064619431577743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGG203.860908E-4108.74828145
AAGCAGT173600.098.837191
AGCAGTG177800.096.4208762
GGTATCA179200.095.7898
GTATCAA179450.095.66869
CAGTGGT178900.095.544264
AGTGGTA179200.095.222445
GTGGTAT179250.095.07456
TGGTATC179950.094.7449657
GCAGTGG180850.094.554163
AGGGGTA1050.082.879535
AAGTCGG406.648354E-572.498856145
GGGTATC1300.066.941167
CAGGGGT1350.064.461864
GAACTAC350.00356180762.14188145
GTAGGCC350.00356180762.14188145
TGTAGTA805.846214E-754.37414145
GGGGTAT1700.051.1903046
AGCAGGG2150.040.476052
GCCAGCT750.001480152238.666054145
Produced by FastQC (version 0.11.5)