FastQCFastQC Report
Sun 19 Mar 2023
SRR4896082_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896082_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504877
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCTAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT131212.598850809206995No Hit
ATGCCTAATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT29470.5837065265401277No Hit
ATGCCTAATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT9180.18182646466367056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCTAA505200.0144.811162
ATGCCTA505250.0144.796831
GCCTAAG180550.0144.670783
GCCTAAT184950.0144.598163
GCCTAAA132000.0143.978423
GCCTAAC9000.0143.301423
CCTAATG101900.0143.276154
CTAATGT50950.0143.062845
CCTAAGG70100.0143.051074
CCTAAGT62200.0143.047744
CTAATGG26150.0142.417865
CCTAAAG55650.0142.177384
CTAAGTG23050.0142.082475
CCTAAGA49800.0141.274224
CTAAATG18050.0140.89745
CTAATAG15800.0140.784335
CTAAGTA16950.0140.636875
CTAAGGA19400.0140.056275
CTAAAGG20400.0139.583925
CTAAGGT22500.0138.793085
Produced by FastQC (version 0.11.5)