FastQCFastQC Report
Sun 19 Mar 2023
SRR4896082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504877
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG280305.551847281615126No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA60491.1981135999461257No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG60181.1919734905729513No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC36830.7294846071419375No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26200.518938276055356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGGCGTGGTGGCTCA11350.22480723027588898No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT9830.1947008875429065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCGGGCGTGGTGGCTCAC7580.15013557757632057No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6780.13429013403264556No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCAGGCGTGGTGGCTCA6020.11923696266615434No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTTTTACCTCGTTGCACTG5360.10616447174262246No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGGCACAGTGGCTCA5260.10418379129966308No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGCAGTAGCTCACG5230.10358958716677527No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCCAGGCGTGGTGGCTCAT5210.1031934510781834No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCCGGACGCAGTGGCTCA5120.10141083867951997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT253400.078.213291
AGCAGTG261950.075.605042
GGTATCA275050.072.2534948
GTATCAA276750.071.9335259
GTGGTAT275200.071.792586
TGGTATC277500.071.275927
CAGTGGT279100.070.855374
AGTGGTA279550.070.7083745
GCAGTGG283150.069.7650763
AAATATA17250.048.751423145
CGTGGGG1601.0095391E-940.77941145
CATGCGG2302.7284841E-1134.67235145
CGGTCTG2353.45608E-1133.934643145
AGGGGTA2206.202754E-1032.9595875
CCTTCGG1703.0088795E-629.851595145
AGAGAGT5450.029.264738145
ACGTACG200.006071647629.00443680-84
GGGGTAT3005.838956E-1026.5847666
AAAATAT26400.026.08784145
GAAACCG12650.026.02065385-89
Produced by FastQC (version 0.11.5)