FastQCFastQC Report
Sun 19 Mar 2023
SRR4896081_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896081_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017907
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGACTATGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC22880.2247749548829117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACT1020000.0144.609071
AAGACTA1020650.0144.516982
AGACTAG346850.0144.510513
GACTATG140550.0144.39194
GACTAGT122350.0144.374454
AGACTAT370650.0144.301423
GACTAGG122300.0143.841054
GACTAAT106050.0143.677644
AGACTAA287500.0143.672553
GACTAGA100550.0143.610374
GACTAAG101750.0143.269644
GACTATA106850.0142.805344
ACTATGG49700.0142.720675
GACTATT122050.0142.532854
ACTAGGT38400.0141.88855
ACTAGAT37500.0141.23645
ACTAGTG40100.0141.113075
CTATGAG12950.0140.990986
ACTAGTA36350.0140.721655
ACTAAGG34600.0140.509935
Produced by FastQC (version 0.11.5)