FastQCFastQC Report
Sun 19 Mar 2023
SRR4896081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017907
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG378473.7181196317541776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88920.873557211022225No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG43890.4311788797994316No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA42350.41604979629769717No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC20270.19913410557153058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAGATGGGTGACCACCT11830.11621886871786911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT557000.0101.6040651
GGTATCA571600.099.148388
GTATCAA573200.098.816179
GTGGTAT569950.098.710356
AGTGGTA571150.098.667975
TGGTATC571950.098.428557
AGCAGTG574800.098.419822
CAGTGGT575200.097.9858554
GCAGTGG580300.097.149683
AGGTATC4150.045.4216547
GGGTATC5500.040.863617
AGGGGTA5600.040.1339045
GAGGTAT4700.040.1063586
AGAGGTA4950.039.5454255
CCGCAAG5700.035.61401145
GGGGTAT6550.034.3129546
TTCGATA2206.220944E-1032.95452145
CAGGGGG6400.031.718729145
CGGTCTG1652.386496E-630.757555145
CGAGTAA950.00472848630.5247969
Produced by FastQC (version 0.11.5)