FastQCFastQC Report
Sun 19 Mar 2023
SRR4896080_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896080_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955772
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT341653.5745972888931674No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT73780.7719414253608601No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT35300.3693349459913034No Hit
CAACCACATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT12080.12638997585198145No Hit
CAACCACATGTACTCTGCGTGGATACCACTGCTTCCCATGTACTCTGCGT10980.11488095487208247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATGT123250.0143.455615
CCACATG194700.0143.176214
ACCACAT408000.0143.042833
CAACCAC972800.0142.385381
ACATGTA101450.0142.354086
AACCACA975550.0141.984012
CCACAGT103000.0141.829964
CACATTG37600.0139.914735
CACATAG32100.0139.281945
CACATGG34400.0138.816685
CACAGTG31500.0138.024235
CACAATG35150.0137.710115
CCACATA104650.0137.654944
CACAGTT32650.0137.379585
CACAGTA33300.0137.091645
CCACATT114400.0136.944414
CCACAAT101650.0136.513644
CACATGA30200.0136.287525
CCACAGG97200.0135.979454
CACAGAT30650.0135.468665
Produced by FastQC (version 0.11.5)