FastQCFastQC Report
Sun 19 Mar 2023
SRR4896080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955772
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG737127.712299586093755No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG166741.7445583256257768No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA105841.1073770731931882No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC92500.9678040369460499No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48260.504932138627204No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA15720.16447437254910166No Hit
AAGCAGTGGTATCAACGCAGAGTACATGTGGTTGAGATCGGAAGAGCACA14420.15087280230013017No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGT10260.10734777750342132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT546450.068.163731
AGCAGTG565300.065.8582462
AAATATA39950.063.87715145
CAGTGGT582200.063.859344
GGTATCA586750.063.6811648
GTATCAA589000.063.4748279
GCAGTGG585800.063.429763
GTGGTAT585850.063.345886
AGTGGTA589850.062.9441835
TGGTATC590450.062.852377
CCGTCGT1401.4149919E-841.426823145
CCGGCTT5900.038.091614145
CATGCGG8850.036.86285145
CATGCGT1905.4278644E-934.340652145
CCGTCTT18600.033.520092145
CGTGGGG4450.030.953747145
AAAATAT59850.028.102406145
CGTGCGG3403.6379788E-1227.719418145
AGCGGTA1658.4176114E-526.3639015
GGTCTGA6050.026.362524145
Produced by FastQC (version 0.11.5)