FastQCFastQC Report
Sun 19 Mar 2023
SRR4896079_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896079_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548496
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGAGG551250.0144.179781
TTGAGGA551450.0144.127492
TGAGGAT200200.0143.72153
TGAGGAG179400.0143.138733
TGAGGAA166950.0142.39863
GAGGATA64750.0138.649234
GAGGATG68100.0137.25514
GAGGAGT68650.0137.10544
GAGGATT75300.0135.678194
GAGGAAT64650.0135.27724
AGGAGTA22600.0134.335715
AGGATTG25350.0133.196825
GAGGAAG57700.0131.227974
AGGATTA22150.0130.849535
AGGATAG20350.0130.673515
AGGATGA20650.0130.178655
AGGATAT23000.0129.79425
AGGAGTG21050.0129.081825
AGGATGT23550.0128.301285
GAGGAGA59950.0128.115754
Produced by FastQC (version 0.11.5)