FastQCFastQC Report
Sun 19 Mar 2023
SRR4896079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548496
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG76951.4029272775006563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37080.6760304541874508No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG31310.5708336979668037No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC22040.4018260844199411No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT11510.20984656223564072No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA10290.18760392053907413No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT6130.11176015868848634No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156200.0109.40078
AAGCAGT155700.0109.306311
GTATCAA156500.0108.995749
GTGGTAT156400.0108.3800356
TGGTATC158300.0107.308217
AGCAGTG159300.0106.735332
CAGTGGT159250.0106.6322564
AGTGGTA160150.0105.942465
GCAGTGG162800.0104.2625053
AAATATA16250.035.689342145
AGGGGTA1551.4622747E-632.745195
AGAGAGT6100.028.522223145
AGGTATC2104.979174E-727.6192747
GAGGTAT2207.4750824E-726.3638536
GGGGTAT1958.744519E-626.0258546
TACGCAC1150.025.21759850-54
CATGGGG43650.024.98118825-29
AAACCGC8550.024.93002985-89
AGAGGTA2351.3274584E-624.6833025
CGCAGAT9050.024.35156890-94
Produced by FastQC (version 0.11.5)