FastQCFastQC Report
Sun 19 Mar 2023
SRR4896078_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896078_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469777
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34420.7326880626339731No Hit
ATCCTGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGTTGAT10060.21414415775995846No Hit
ATCCTGTAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT7670.16326895527026652No Hit
ATCCTGTATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6950.1479425344365518No Hit
ATCCTGTACTCTGCGTTGATACCACTGCTTCGCACGTACTCTGCGTTGAT6320.13453191620705143No Hit
ATCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6080.12942310926247985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGTA470700.0144.610892
ATCCTGT471050.0144.503451
CTGTAGG49250.0144.462024
CCTGTAG146800.0144.459173
CTGTATG56900.0143.757374
CCTGTAT168000.0143.738953
CTGTAAG43350.0143.56634
CCTGTAA133200.0143.543523
CTGTAGT58450.0143.41594
CTGTATA48300.0142.353294
CTGTAGA38650.0141.904074
CTGTAAT54000.0141.817444
CTGTATT63700.0139.671364
CTGTAAA36800.0138.80014
TGTATAG16700.0137.961885
TGTAGTA16350.0137.370165
CTGTACT19950.0136.913684
CCTGTAC26500.0135.607673
TGTAGTG20000.0135.122385
TGTATGA16300.0134.680115
Produced by FastQC (version 0.11.5)