FastQCFastQC Report
Sun 19 Mar 2023
SRR4896078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences469777
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG297626.335346345180798No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG53021.12862060083827No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA43910.9346988039005741No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40430.8606211032042862No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC23450.4991730118758475No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA6860.14602673183233747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT411250.099.7476041
GTATCAA420000.097.631479
AGCAGTG422450.096.890262
CAGTGGT422450.096.564094
GGTATCA424450.096.457628
GTGGTAT422550.096.393676
TGGTATC423600.096.2232067
AGTGGTA423850.096.1493455
GCAGTGG430050.094.9587553
AAATATA19200.050.597992145
AGCGGTA1002.727391E-643.5084345
CGGTATC1002.727391E-643.5084347
GGCACTA700.001056167841.42778145
CCGCTAG750.001482724138.665928145
ACGTCGG750.001482724138.665928145
GCGGTAT1157.125147E-637.8334166
TGATAGG1251.2638278E-534.79934145
CATCGGC1051.8524317E-434.52315145
AGGGGTA4300.033.7274675
CGGTCTG900.003626627432.221607145
Produced by FastQC (version 0.11.5)