FastQCFastQC Report
Sun 19 Mar 2023
SRR4896077_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896077_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545298
Sequences flagged as poor quality0
Sequence length151
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACTA545700.0144.781562
AGTCACT545800.0144.755041
TCACTAG161750.0144.632313
TCACTAA236150.0143.949983
CACTAAG70650.0143.875564
CACTATG45300.0143.621584
CACTAGG45300.0143.621584
TCACTAT141950.0143.420913
CACTAGA95650.0143.234654
CACTAGT21150.0142.845724
ACTATAG27700.0142.808625
CTATAGA16700.0142.731896
CACTATA83200.0141.853264
ACTAAAG42400.0141.654455
CTATAAG17800.0141.644856
CACTAAA139100.0141.56764
ACTATGA24200.0141.308465
ACTAGGA26800.0140.8465
ACTAGAA57100.0140.460135
CACTAAT27900.0139.447784
Produced by FastQC (version 0.11.5)