FastQCFastQC Report
Sun 19 Mar 2023
SRR4896077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545298
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG158692.9101518802563002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39940.7324435446306423No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG39530.7249247200613242No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC21540.39501336883685617No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA20150.3695227196872169No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACACAGGATCCGGAGCTGGT10490.19237187739547917No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT6960.12763663171330172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT327000.0110.76511
GGTATCA332800.0108.8682868
GTATCAA333050.0108.711299
GTGGTAT333700.0107.988016
AGCAGTG336300.0107.649022
TGGTATC334950.0107.606667
CAGTGGT336400.0107.2075044
AGTGGTA337800.0106.7417145
GCAGTGG340750.0105.838893
AGGGGTA2300.050.439125
ACGTTAA450.009637020548.333057145
AGCGGTA750.001482167338.6699945
AGGTATC2302.5465852E-1134.6768957
GGGTATC3350.034.6298457
TGCGGCT2455.4569682E-1132.550835145
GCGGTAT900.00362526832.2249956
CGGTATC900.00362526832.2249957
GGGGTAT3700.031.3540486
AAATATA21150.027.765799145
ATGGGGG34400.026.21787325-29
Produced by FastQC (version 0.11.5)