FastQCFastQC Report
Sun 19 Mar 2023
SRR4896076_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896076_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038769
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGGA2045000.0144.46991
GCAGGAA2046900.0144.33582
CAGGAAT777100.0144.157553
CAGGAAG626600.0143.224533
CAGGAAA632800.0140.332753
AGGAATG260250.0139.455934
AGGAATA248850.0138.91484
AGGAAGT241200.0138.634454
AGGAATT291600.0137.632354
AGGAAAT254000.0136.240834
GGAATAG80050.0134.865745
GGAATTG98100.0134.720155
GGAATGA74400.0133.713215
GGAAGTA73700.0132.42715
GGAAGTG75850.0132.39895
GGAATGT93950.0132.341865
GGAATTA84000.0131.456625
AGGAAGG245000.0130.983414
GGAATAT94700.0130.37565
AGGAAGA180500.0130.341224
Produced by FastQC (version 0.11.5)