FastQCFastQC Report
Sun 19 Mar 2023
SRR4896076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038769
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG259441.2725325919709394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180000.8828857021074973No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG47520.23308182535637925No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA34970.17152507223721766No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC29160.14302748374141455No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAGGGCACGGTGGCTCATG26080.12792032839424183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT890450.0122.4854351
GGTATCA906750.0120.452768
GTATCAA910900.0119.860399
AGTGGTA904600.0119.844295
TGGTATC906700.0119.563797
GTGGTAT906500.0119.518196
AGCAGTG913400.0119.194372
CAGTGGT913700.0118.750454
GCAGTGG930900.0116.595273
GAGGTAT8650.056.159436
AGGTATC9000.053.169857
CGGTATC2850.050.880247
GCGGTAT3050.047.543836
GGGTATC8100.044.7557647
AGAGGTA11000.044.1628235
AGCGGTA3450.042.032535
AGGGGTA9100.039.041765
GGGGTAT9400.037.7948156
ACTAAAG16500.026.362627145
CAGCGGT5550.026.1328184
Produced by FastQC (version 0.11.5)