FastQCFastQC Report
Sun 19 Mar 2023
SRR4896075_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896075_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262891
Sequences flagged as poor quality0
Sequence length151
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT44251.6832071086495926No Hit
AGATCGCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT6130.23317648759371754No Hit
AGATCGCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT4460.1696520611203883No Hit
AGATCGCAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT3840.14606814230993073No Hit
AGATCGCATGTACTCTGCGTTGATACCACTGCTGCCCATGTACTCTGCGT2800.1065080204343245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG100.0071009942144.910579
CATGCAG100.007105035144.883017
CAGTCGA351.0913936E-11144.883017
AGTACTG151.2329397E-4144.883018
GATCGCA263100.0144.745352
AGATCGC263350.0144.607931
TCGCAGT31400.0144.42164
ATCGCAA69600.0144.154433
ATCGCAT100700.0144.091693
TCGCAAT26650.0144.067544
ATCGCAG86100.0144.041643
CGCATAG8150.0143.994165
TCGCAAG20900.0143.149964
TCGCATA25050.0142.85874
CGCAGTG10500.0142.813255
TCGCATG43550.0142.72064
CGCATTG13150.0142.679475
TCGCATT32150.0142.629794
TCGCAAA21700.0142.212374
TCGCAGA23400.0142.09684
Produced by FastQC (version 0.11.5)