FastQCFastQC Report
Sun 19 Mar 2023
SRR4896075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262891
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG201627.669338242845894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31941.21495220452583No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA29991.1407769760090685No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA9830.3739192288819321No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG7710.29327744198165784No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6750.25676040640417513No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCACGGTGGCTCATG5660.21529835559224164No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCCAGGCGCGGTGGCTCATG5460.20769063984693278No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5190.19742022359076575No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA4760.18106363473835163No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGTGAAACCCCATCTCTA3810.14492698494813439No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGAGGGCAAGTCTGGTG2750.10460609149799728No Hit
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2640.1004218478380774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT243750.096.0890661
AGCAGTG250750.093.204192
AGTGGTA250350.092.94765
GGTATCA252950.092.823568
GTATCAA253250.092.7245949
GTGGTAT252250.092.1325156
CAGTGGT253550.091.917534
TGGTATC253150.091.833617
GCAGTGG255650.091.247573
GCGGTAT801.8189894E-1281.577166
AGCGGTA851.8189894E-1276.77855
CGGTATC851.8189894E-1276.77857
GGGTGTA502.301651E-672.49924145
CGTGCGC808.056304E-963.436836145
CAGCGGT1102.5465852E-1159.3288424
CCGTAAT400.00604619554.37443145
GGGGTAT1750.053.8668256
GGGTATC1800.052.3705257
AGGGGTA1950.048.3420225
GTTAGGC450.00963169248.33283145
Produced by FastQC (version 0.11.5)