FastQCFastQC Report
Sun 19 Mar 2023
SRR4896074_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896074_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681504
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTCA682250.0144.757491
GAGTCAA682550.0144.693882
AGTCAAG226550.0144.309113
AGTCAAT250950.0144.13723
GTCAATG87000.0143.750814
GTCAAGA67750.0143.526464
AGTCAAA198400.0143.455953
GTCAAGT78800.0143.445564
GTCAATA72300.0143.31334
GTCAAGG79650.0143.279314
GTCAATT91600.0142.781024
GTCAAAG72400.0142.31474
GTCAAAT74600.0142.197174
TCAATAG24000.0138.87865
TCAATGG31200.0137.949665
TCAATTG32550.0137.79345
GTCAAAA53900.0137.791954
TCAAGAG21400.0137.46785
CAAGTTG8400.0137.15346
CAATGTG11750.0136.283466
Produced by FastQC (version 0.11.5)