FastQCFastQC Report
Sun 19 Mar 2023
SRR4896074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681504
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG131971.9364523172277788No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG98221.4412241160726864No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52470.7699147767291168No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC38580.5661008592759543No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA24670.3619934732591445No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA24340.35715124195896136No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA17640.25883927313706157No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGAAACTCCCTGTAAACT12380.181657040897779No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT12210.1791625581067756No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTTAGTTTGTGGCTTCAT10410.15275038737850402No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAATAGAAATTTATGTAG10330.15157651312391415No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGAAGAAATGCAAACTAT9700.1423322533690191No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTTGAGTCTGGAAATAACT8580.12589801380476123No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA8020.11768089402263229No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTGGTTGAATGTGTATCTA7600.1115180541860356No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATCGGCGTAAATCTAACTTT7150.1049150115039677No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTCTATGTTCATTCTATA7120.1044748086584965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT321950.0115.7200241
GGTATCA330650.0113.053368
GTATCAA330750.0112.835559
AGCAGTG329850.0112.750652
GTGGTAT329600.0112.555666
TGGTATC330950.0112.052717
CAGTGGT331950.0111.797074
GCAGTGG336600.0110.3172763
AGTGGTA336700.0110.182195
CGCCGAA406.1179526E-790.61858145
AGGGGTA3300.063.7122845
CAGGGGT4150.050.6702164
GGGGTAT4150.050.6627776
GGGTATC4350.050.0001267
AAATATA26750.049.865627145
CGATAAG803.7349557E-545.30929145
AGTACGC700.001056772341.425636145
AGGTATC3000.036.250097
AGCAGGG6800.033.0564042
GTATCAC4400.031.30469
Produced by FastQC (version 0.11.5)