FastQCFastQC Report
Sun 19 Mar 2023
SRR4896073_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896073_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466830
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAG160450.0144.748343
ACGTATC467350.0144.713271
CGTATCA467650.0144.620442
TATCAGT55900.0144.365424
GTATCAA131350.0143.946213
GTATCAT168950.0143.726093
TATCAGA44050.0143.40374
TATCAAG47750.0143.366674
TATCAGG60450.0143.325884
TATCATG58050.0142.887124
ATCATAG17200.0141.935585
ATCAGGT20350.0141.679965
ATCAGGA16450.0140.92045
ATCAGTA16700.0140.545945
ATCAGTG18050.0140.469045
ATCATGA15650.0140.254915
ATCATGG19500.0140.054325
ATCAGTT18250.0139.723545
ATCATGT20300.0139.530945
ATCAGAG13050.0139.332695
Produced by FastQC (version 0.11.5)