FastQCFastQC Report
Sun 19 Mar 2023
SRR4896073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466830
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG93111.9945162050425207No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41820.8958293168819486No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA12490.267549214917636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG11790.25255446308077883No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT7770.1664417453891138No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC6080.13024013024013026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT176500.0107.740681
AGCAGTG179850.0105.5952152
GGTATCA181600.0104.8068
AGTGGTA180900.0104.4099355
GTATCAA182450.0104.374849
GTGGTAT181450.0104.1334156
CAGTGGT182650.0103.5794454
TGGTATC182800.0103.404037
GCAGTGG185950.0101.819253
ACGTATC1450.059.9983631
CATCGGA400.006049527354.373512145
AGCGCAT1001.3905362E-436.24901145
TAATCCG800.00203577836.24901145
CGTATCA2353.45608E-1133.9425162
CGGTCTG1501.1324137E-633.83241145
AGGGGTA1751.0065196E-733.14555
AGGTATC2505.456968E-1229.0023167
GGGTATC2003.2457865E-729.0023167
GAGGTAT2502.4374458E-929.0023166
TTAGCGC200.006073727729.00231240-44
Produced by FastQC (version 0.11.5)