FastQCFastQC Report
Sun 19 Mar 2023
SRR4896072_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896072_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422715
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACATCG423500.0144.65032
ACATCGG115050.0144.42023
ACATCGT170650.0144.41453
ACATCGA112400.0144.085973
ATCGAGT13300.0143.83445
CATCGTG51700.0143.802784
AAACATC426900.0143.498251
ATCGGGA10100.0143.489175
CATCGTT78450.0143.076724
CATCGGT47350.0142.93464
TCGAGTA3550.0142.882876
ATCGGTA12650.0142.632775
CATCGAT45000.0142.508674
CATCGTA40150.0142.397354
CATCGAG40000.0142.387884
CATCGGG40650.0142.071934
CATCGGA26800.0141.40914
ATCGATG13350.0141.124545
ATCGTGT19150.0140.761755
ATCGTAG12400.0140.24915
Produced by FastQC (version 0.11.5)