FastQCFastQC Report
Sun 19 Mar 2023
SRR4896072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422715
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG169224.003169984504926No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29850.7061495333735496No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA23170.5481234401428858No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG18190.43031356824337913No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC10030.23727570585382585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT197450.092.373221
GTATCAA200900.090.790799
AGTGGTA201050.090.398525
AGCAGTG201650.090.355942
GGTATCA202550.090.2659458
GTGGTAT202300.089.8041156
CAGTGGT202650.089.74184
TGGTATC204100.089.118687
GCAGTGG205700.088.481663
CGGTCTG1002.7315436E-643.497303145
ACATCGT750.001481144638.673423
AACATCG1157.1301765E-637.8282172
TTCGATA900.003626980632.220226145
CGACCTA353.776273E-629.00094845-49
AGGGGTA2003.2485877E-728.9982015
GGGGTAT2054.0320265E-728.290936
GGGTATC2351.3281206E-624.679327
CCGCAAG2502.2752683E-623.198563145
ATGGGAT25400.022.71852725-29
CATGGGG31100.022.37750425-29
Produced by FastQC (version 0.11.5)