FastQCFastQC Report
Sun 19 Mar 2023
SRR4896071_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896071_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285094
Sequences flagged as poor quality0
Sequence length151
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCTCGAAGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC3770.13223708671525883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGT151.231937E-4144.915829
GATTGCA151.231937E-4144.915827
ATACGGT151.231937E-4144.915828
GATTCGA306.511982E-10144.915827
TCGACTA600.0144.915825
GATAGCT253.7174686E-8144.91587
CGCTCGA285300.0144.788822
ACGCTCG285350.0144.763441
GCTCGAA80900.0144.736683
GCTCGAT99350.0144.624073
GCTCGAG98950.0144.62293
CTCGATA30300.0144.198414
CTCGAAT29800.0144.186374
CTCGAGA29400.0144.176444
TCGAAGT9350.0144.140855
CTCGATT36250.0143.916384
CTCGATG32700.0143.807894
CTCGAAG28050.0143.624224
TCGAATA7800.0143.05795
CTCGAGT33950.0142.994984
Produced by FastQC (version 0.11.5)