FastQCFastQC Report
Sun 19 Mar 2023
SRR4896071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285094
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG111463.9095877149291107No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25360.8895311721747914No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT15350.5384189074480698No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA13930.48861077399033304No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA9130.32024525244305385No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG6810.23886858369520225No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTTCAACTTTAAATTTGCC5600.196426441805159No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGTGGGAAGATTTATAGGTA5410.18976197324391253No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGAAGAAATGCAAACTAT4700.16485790651504414No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGCAAAATAGTGGGAAGATT4260.14942440037321023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4140.14521526233452828No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC4060.14240917030874026No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTCTATGTTCATTCTATA3370.11820662658631889No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA3250.11399748854763693No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGA3140.11013911201217845No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGAATAGAAATTTATGTAG2910.10207159743803797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAAACCTAGCCCCAAACCC2910.10207159743803797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCC151.2291552E-4144.99806145
AAGCAGT181700.0105.3555451
AGTGGTA186400.0102.0976265
GTATCAA188500.0101.575579
CAGTGGT187750.0101.458524
GGTATCA188350.0101.4255148
AGCAGTG188150.0101.31992
GTGGTAT187900.0101.2054146
TGGTATC188700.0100.8147667
GCAGTGG190450.099.944013
TATCGGT406.655464E-572.49903145
AATCGAG350.003564077262.14203145
TATGCCC606.7936944E-660.415863145
AGGGGTA1152.4738256E-950.4341135
GGGTATC1502.5886038E-838.6661537
AGAGAGT5550.032.657223145
CGCTGTT900.003624151232.221794145
GGGGTAT1801.2870441E-732.2217946
CAGTTAG950.00472199530.525911145
AGAGGTA1204.0511577E-430.2079325
Produced by FastQC (version 0.11.5)