FastQCFastQC Report
Sun 19 Mar 2023
SRR4896070_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896070_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404784
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTCCAATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT38200.9437131902446737No Hit
ACCTCCAATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGT6280.15514447211352225No Hit
ACCTCCAATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT4200.10375904185936204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCCA408650.0143.529561
TCCAAGT46850.0143.365984
TCCAATG55850.0143.355744
CCTCCAA410500.0142.88272
CTCCAAT148300.0142.762793
CTCCAAG137350.0142.48593
TCCAAAT42950.0142.044654
CCAATAG14700.0141.462235
CTCCAAA117600.0141.030943
CCAATGT25800.0140.98085
TCCAAGA41600.0140.55824
CAAGATG4350.0139.915566
TCCAATA44400.0139.037724
TCCAATT49000.0138.702014
CCAAGAT14500.0138.416465
TCCAAGG48600.0138.203634
TCCAAAG40900.0138.180664
CCAAGTG14650.0137.49385
CAATGTA15250.0137.310566
CCAATGA13750.0137.008225
Produced by FastQC (version 0.11.5)