FastQCFastQC Report
Sun 19 Mar 2023
SRR4896069_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896069_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630262
Sequences flagged as poor quality0
Sequence length151
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGA1634000.0144.584122
ACAGCAG1634300.0144.557591
AGCAGAT571150.0144.472673
GCAGAGT187800.0143.797794
GCAGATG191750.0143.783074
GCAGATT192900.0143.714694
AGCAGAG536650.0143.634023
GCAGATA183250.0143.335074
GCAGAAT178450.0143.251924
AGCAGAA506650.0143.028923
CAGATAG61050.0142.305595
CAGATTG66650.0141.981415
CAGATAT61800.0141.047565
GCAGAAG177900.0141.006644
GCAGAGA163750.0140.801514
CAGATGA54750.0140.681695
CAGATGT63300.0140.681375
CAGATGG65450.0140.599085
CAGAATG61000.0140.521685
CAGAAGT57550.0140.258215
Produced by FastQC (version 0.11.5)