FastQCFastQC Report
Sun 19 Mar 2023
SRR4896069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630262
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG200681.2309677830925336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131930.8092564262676797No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTGAGGCAGCCAGCG65390.40110117269494105No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC27550.16899124189854145No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA26380.16181448135330395No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAGAAATGCAAACTATCA22610.13868936404087195No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGTCTATGTTCATTCTATA22450.13770792670135232No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATTCTATAGAAGAAATGCAA19460.11936731641907865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT612650.0118.875651
GGTATCA621200.0117.446858
GTATCAA624250.0116.784579
GTGGTAT621400.0116.674016
AGTGGTA622850.0116.440885
TGGTATC623850.0116.3668757
AGCAGTG625400.0116.2630542
CAGTGGT629250.0115.286694
GCAGTGG633150.0114.5994643
GAGGTAT5350.046.0755466
AGGGGTA5350.042.0113455
GGGTATC5250.041.429277
AGGTATC5850.040.898137
AGAGGTA7150.034.4771655
CGCAAGG5550.027.431213145
CGGTCTG3750.027.065468145
GGGGTAT8750.026.5147346
ATGGGAT130600.025.97925825-29
AGCGGTA1701.0313495E-425.5894555
CGCTGTT6050.025.164171145
Produced by FastQC (version 0.11.5)