FastQCFastQC Report
Sun 19 Mar 2023
SRR4896068_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4896068_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1501632
Sequences flagged as poor quality0
Sequence length151
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACAGA1516000.0143.51551
CAGAAGT172550.0143.506264
CACAGAA1518250.0143.302812
ACAGAAT537700.0143.234573
CAGAATG189200.0143.130424
CAGAATT183500.0142.917794
ACAGAAG496050.0141.883263
CAGAAAT167100.0140.469594
CAGAAGG177550.0139.750584
CAGAATA163700.0139.53694
CAGAAAG167550.0137.152114
CAGAAGA147800.0135.52974
ACAGAAA482300.0133.686193
AGAAGGT55800.0130.220845
AGAATTG64850.0129.922245
AGAAGTG52500.0128.74675
AGAATGG59750.0128.523255
AGAATGT62200.0128.236225
AGAAGTC27900.0128.01375
AGAAGTT55350.0127.74565
Produced by FastQC (version 0.11.5)